phenyl sepharose cl-4B

phenyl sepharose cl-4B - Names and Identifiers

Name	Phenyl Sepharose CL-4B
Synonyms	PHENYL SEPHAROSE Phenyl-Sepharose?CL-4B Phenyl Sepharose CL-4B phenyl sepharose cl-4B phenyl-sepharose(R) cl-4B PHENYL SEPHAROSE 6 FAST FLOW Sepharose CL 4B, phenyl ether Phenyl-Sepharose(R) 6 Fast Flow PHENYL-SEPHAROSE(R) CL-4B, FOR AFFINITY CHROMATOGRAPHY*
CAS	69106-59-8

phenyl sepharose cl-4B - Physico-chemical Properties

Flash Point	36°C
Appearance	Containing 20% ethanol, colloid at the bottom
Storage Condition	2-8°C

phenyl sepharose cl-4B - Risk and Safety

Hazard Symbols	Xi - Irritant
Risk Codes	R10 - Flammable R36/37/38 - Irritating to eyes, respiratory system and skin.
Safety Description	S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S16 - Keep away from sources of ignition.
UN IDs	UN 1993 3/PG 3
WGK Germany	2
FLUKA BRAND F CODES	10

phenyl sepharose cl-4B - Introduction

Phenyl Sepharose CL-4B (Phenyl Sepharose CL-4B) is a commonly used adsorbent for the separation and purification of protein molecules. It is a hydrophilic gel prepared by introducing phenyl functional groups on a polyvinyl agarose (Sepharose) substrate.

The gel has good chemical stability, mechanical strength and large specific surface area. The phenyl functional group makes it highly hydrophilic and hydrophobic, and can interact with protein molecules through hydrogen bonds, van der Waals forces and hydrophobic interactions. This interaction can be used to separate and purify proteins with different hydrophobic and hydrophilic properties.

The main use of Phenyl Sepharose CL-4B is to purify and enrich proteins in biochemical experiments. It can be used to isolate and enrich proteins of a particular hydrophilic hydrophobic nature, for example to enrich hydrophobic proteins by hydrophobic interactions. It can also be used in affinity chromatography, affinity purification and affinity dialysis for the separation and purification of protein complexes with other small molecules.

The method for preparing Phenyl Sepharose CL-4B is to react the phenyl functional group with polyvinyl agarose, and introduce the phenyl group into the agarose matrix. This preparation method allows the phenyl functional groups to be uniformly distributed on the agarose matrix.

When using Phenyl Sepharose CL-4B, you need to pay attention to some safety matters. Avoid contact with skin and eyes, wear appropriate protective gloves and glasses when using. Follow the laboratory's safe operating procedures during operation and properly dispose of waste.

Last Update：2024-04-09 19:06:00